ASTM D7463-14 PDF

1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with:

1.1.1 Microorganisms found in conventional liquid fuels with kinematic viscosities (at 40°C) of ≤ 8 mm² · s-1 as described in Table X6.1,

1.1.2 Microorganisms found in fuel-associated bottom water, and

1.1.3 Extracellular (non-cellular) ATP present in the sample matrix.

1.2 The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens2 and a dedicated luminometer2 and reported in (instrument specific) Relative Light Units.

1.3 This test method is equally suitable for use in the laboratory or field.

1.4 Although bioluminescence is a reliable and proven method for qualifying and quantifying ATP, this method does not differentiate between ATP from different sources, for example, from different types of microorganism such as bacteria or fungi.

1.5 For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 × 10-11 M to 3 × 10-8 M which is equivalent to 1 × 10-14 moles/mL to 3 × 10-11 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 × 10-11 M to 2 × 10-14 M.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.