The objective of this slide presentation was to evaluate enumeration methods for the detection of
coliform bacteria in water samples using a precise number
of organisms. Escherichia coli (ATCC = 11775) and Enterobacter
aerogenes (ATCC = 13048) BioBall™ cultures were used.
All methods were tested 10 times using each culture and
the mean number ¿¿¿¿ SD of all readings was calculated. Eight methods were evaluated using BioBall™ cultures:
membrane filtration;
mFC and mLS;
defined substrate technology systems;
Colilert™ and Colisure™
multiple tube fermentation (MPN);
Petrifilm;
standard plate count; and,
pour and spread plate method. Results of the methods are given, and conclusions were that:
Petrifilm and PCA-P produced the most accurate and
consistent results(1 mL);
Colilert produced the most consistent results with the lowest
coefficient of variation (100 mL);
MF, Colisure and MPN may be producing results with
greater variability; and,
BioBall™ is a very useful quality control tool for evaluating
the efficiency of water testing procedures. Includes figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 1 MB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 38 |
| Published : | 06/01/2006 |
