Conventional cell culture methods for the detection of enteroviruses (EV) and the Hepatitis A virus (HAV) in the environment are expensive, time consuming and may not detect noncytopathogenic strains. Direct polymerase chain reaction (PCR) is a rapid, sensitive alternative but it is often complicated by inhibitory factors and small reaction volumes. An integrated cell culture/PCR (ICC/PCR) approach has been developed that provides a rapid detection method for viable and noncytopathogenic viruses in PCR inhibitory samples. The objective of this study was to develop a rapid method for the detection of viable HAV and EV in environmental samples. Optimization of ICC/PCR for cytopathogenic and noncytopathogenic viruses may lead to a timely and cost effective method for routine monitoring of environmental samples for the presence of human viruses.
| File Size : | 1
file |
| ISBN(s) : | 0898679583 |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 8 |
| Published : | 01/01/1997 |
