Escherichia coli O157:H7 serves as the etiological agent for hemorrhagic colitis,non-bloody diarrhea, and the hemolytic uremic syndrome. E. coli O157:H7 hasemerged as an important waterborne bacterial pathogen. Recent outbreaks havecentered attention on the role of drinking water in the transmission of thisorganism. It has been shown that the pathogen is sensitive to chlorination and issimilar in resistance to generic strains of E. coli, however, it continues to bea problem in untreated drinking water systems. As with any microbial pathogen,the ability to rapidly detect the presence of the pathogen may greatly enhancethe ability of public health authorities to respond in an outbreak. This studywas designed to evaluate a rapid method for the detection of E. coli O157:H7 inwater. A flow cytometric instrument was used to detect the presence of theorganism directly from water samples and after pre-enrichment and immunomagneticseparation. Affinity purified fluorescently labeled polyclonal anitbodiesspecific for E. coli O157:H7 were used in the study. Includes tables, figure.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 200 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 5 |
| Published : | 01/01/2000 |
