The main objectives of this study were to develop methods to isolate microsporidia from water
and, once they were isolated, to determine the most efficient and accurate method to
identify the recovered microsporidia. Identification techniques included microscopic morphology,
immunological reactions, and molecular assays such as PCR. The only US Environmental Protection Agency (USEPA) approved method
for recovery of protozoa from water (Method 1623; USEPA 1999) involves filtration of a 10 L
water sample followed by elution from the filter, concentration by centrifugation,
immunomagnetic purification of target organisms and visualization by an immunofluorescence
assay. Since this laboratory routinely obtains high recovery efficiencies with low coefficients of
variation for Cryptosporidium and Giardia using this method, it was used as the basis for
developing microsporidia recovery methods. A variety of filtration methods were tested using
several different filter technologies with commercially available and custom-made filters. A
variety of fluorescent and non-fluorescent stains were tested for morphological identification and
many primers and probes were tested for their specificity and affinity to DNA from different
species of microsporidia.
Includes 8 references, table.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 250 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 7 |
| Published : | 11/01/2002 |
