A quantitative method to detect infectious enteroviruses in water by integrated cell culture-PCR
(ICC-PCR) was evaluated. Of 9 water samples, 7 were positive for enteroviruses using ICC-PCR.
The concentrations of enteroviruses were calculated by the Most Probable Number (MPN) method
and ranged from 33 to 189 MPN/100 L. ICC-PCR-MPN is a very sensitive and rapid method to
quantify infectious enteroviruses in water. Male-specific coliphages (F+) were detected in water
samples using single agar layer (SAL) and direct membrane filtration (DMF). Double agar layer
(DAL) method was used to detect F+ coliphages in concentrated water samples. All samples were
positive for male-specific coliphages, ranged from 3 to 180 pfu/100 mL. Correlation (r = 0.61)
existed between the presence of enteroviruses and male-specific coliphages in concentrated water
samples. A total of 61 male-specific coliphage isolates were serotyped to determine their
serogroups. The results indicated that 74% were RNA phages with the majority being groups I and
II. However, group III male-specific coliphages were detected in all sites, indicating human
sources were likely to be contributing some of the fecal contamination. Includes 12 references, tables.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 330 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 8 |
| Published : | 11/01/2002 |
