The goal of this research was to evaluate the parameters influencing the degradation of
cylindrospermopsin by chlorine. Sodium hypochlorite was the source of chlorine used
for the evaluation, with cylindrospermopsin obtained from a cell free extract of culture
material. All experimental samples were of 100 ml volume in Milli-Q water, and
undertaken in sealed Schott bottles. Prior to chlorination and after a given reaction
period, 1 ml sub-samples were collected and immediately filtered through 0.45 um filters
into a vial containing 25 ul acetic acid. Acetic acid was used to quench chlorine from
samples for HPLC-MS/MS analysis of cylindrospermopsin as per method of Eaglesham
et al. (1999). At the same time a 25 ml sample was collected for free chlorine
determination using DPD pillows (Hach, 1993). Includes 8 references, tables, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 360 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 14 |
| Published : | 11/01/2002 |
