This study designed real-time PCR assays which amplify Cryptosporidium parvum sequences with
multiple nucleotide differences between type 1 and type 2. The presence of single-nucleotide
polymorphisms affected the amplicon melting temperature and the melting temperature of
internal fluorescent probes, the basis for a type-specific diagnostic assay. By combining two
pairs of PCR primers, one specific for Cryptosporidium parvum the other for Giardia lamblia, a
multiplex assay for detecting both organisms was developed.
Includes 17 references, table, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 570 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 8 |
| Published : | 11/01/2002 |
