Conventional culture methods for the detection of Legionella species often require
filtration and centrifugation to concentrate target organisms and subsequent
decontamination prior plating on highly selective agar media. Aliquots of sample
concentrates may subsequently be treated by heat or acid to reduce or eliminate high
levels of non-target organisms that may be present in the sample. These treatments
have been shown to reduce the total viable Legionella counts (TVLC).
Immunomagnetic separation, utilizing beads coated with antibodies to a specific
target organism, has been shown to be an effective strategy for isolating target
organisms from complex environmental, food and clinical samples (Riffard et al.,
2001; Yanez et al., 2005). This approach may eliminate the need for sample pretreatments
and reduces the need for highly selective culture medium.
The purpose of this evaluation was to compare the performance of Dynabeads® anti-
Legionella using and an automated immunomagnetic separation protocol using the
Bead Retreive™ with a standard protocol for the isolation and quantification of
Legionella pneumophila in environmental samples. Includes tables.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 160 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 4 |
| Published : | 11/01/2005 |
