This study was initiated to determine the usefulness of Coli ID agar in
recovering chlorine-stressed E. coli and other coliform organisms from drinking
water and to compare the results with Tergitol-TTC agar stipulated by European
Guideline 98/83 as a reference method. Moreover, the Colilert-18 /Quanti-Tray
medium was used. To that end, the study analyzed marginally chlorinated water
samples by means of the most probable number method using Colilert-
18 /Quanti-Tray, and the membrane
filtration technique using Coli ID and TTC-Tergitol agar, according to ISO 9308-
1:2000 method for coliforms and E. coli. Variance analysis of the data obtained
showed statistically significant differences in the coliform counts "confirmed" on
Coli ID compared with this of reference method used. The coliform counts on
Coli ID from marginally chlorinated water samples were significantly greater
(P<0.01) than the corresponding values of the Tergitol-TTC agar and
significantly less (P<0.05) than that of Colilert-18 /Quanti-Tray. In this same
water, variance analysis of the data also demonstrated the absence of
statistically significant differences in the E. coli counts "confirmed" of Coli ID
compared with Tergitol-TTC and Colilert-18 /Quanti-Tray. In summary, the Coli
ID agar could be used as an alternative method to reference method for detecting
and recovering E. coli and other coliform bacteria in treated water. However,
Colilert-18 /Quanti-Tray was the most suitable for recovering other coliform
bacteria chlorine-stressed non E. coli organisms. Includes 10 references, tables, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 230 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 9 |
| Published : | 11/01/2005 |
