Simple, rapid and reliable fecal indicator tests are needed to better monitor and
manage the fecal impacts and sanitary quality of waters with human uses. The
new US Environmental Protection Agency (USEPA) Ground Water Rule uses coliphages and other fecal indicators (E.
coli, enterococci) to monitor the quality of groundwater used in public water
systems. This study addressed needs of the Ground Water Rule by creating a
rapid and simple 3-hour microbial water quality monitoring system for F+
coliphages as fecal indicators. Water samples are processed by a 3-hour F+ coliphage culture
enrichment which is a modified version of USEPA method 1601 for groundwater. The research hypothesis was that modifying USEPA method 1601 by
increasing initial E. coli levels in enrichments would decrease the total coliphage
culture time from 16-24 hours to 60-360 minutes. This development and evaluation of a rapid F+ coliphage culture enrichment and
novel immunological agglutination test provides a new tool for same-day
monitoring and decisions on the microbiological quality of water. This work also
improves access to F+ coliphage detection and source tracking by making it
simpler, as affordable as bacteriological analysis, rapid, and potentially field-portable.
This new coliphage method can be applied to monitor groundwater
quality under the USEPA Ground Water Rule, which affects about 150,000
public water systems that use groundwater and other mixed groundwater/surface
water systems. Includes 15 references.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 74 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 5 |
| Published : | 11/01/2007 |
