An integrated on-line SPE-HPLC-MS/MS system has been developed for rapid analysis of
various groups of perfluorinated compounds (PFCs) in surface and drinking water. 17 PFCs were
included in this study, with various carboxylic, sulfonate, and sulfonamide species among them.
Use of Lower Pressure Turbulent-flow chromatography columns (LP-TFC) as solid phase
extraction (SPE) cartridges enables fast on-line SPE at high sampling flow-rate (3-5 ml/min).
Polymeric Oasis hydrophile-lipophile balance (HLB) LPTFC column allow complete extraction with good recoveries from
preserved water. On-line coupling to high pressure liquid chromatography (HPLC) is performed with re-mixing of the organic LPTFC
eluate with water in front of the analytical column to ensure efficient band focusing or a rapid
increase and decrease of organic solvent gradient can be used instead for efficient band focusing.
For fast HPLC analysis, a short C18 column with 1.8 µm particle size is applied in combination
with highly selective API-MS/MS detection. Matrix effects on electro spray ES-MS-/MS signal
were found to be adequately corrected by using isotopic labeled standards and or using standard
addition technique. Limits of detection, determined for 1-ml sample of source water were in the
range between 1 to 5ng/L typically for carboxylic and sulfonate PFC groups with chain length of
C4-C9, and about 20ng/L for sulfonamide and C10-C14 PFCs. At an enriched water volume of
1 ml, the whole SPE-HPLC-MS/MS procedure requires less than 10 minutes. The method was
successfully applied to the analysis of drinking and source water samples for most of PFCs, the
longer chain PFCs C10-C14 needs further refinements. Includes 15 references, tables, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 570 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 16 |
| Published : | 11/01/2007 |
