This document describes a method for the determination of individual ergot alkaloids and tropane
alkaloids in unprocessed cereals and cereal-based compound feeds by high performance liquid
chromatography with tandem mass spectrometry (LC-MS/MS).
This document has been successfully validated by collaborative trial in the following matrices: rye, barley,
wheat, complete feed for bovine, porcine and poultry. Validation in buckwheat produced acceptable
results, but the relative standard reproducibility was higher for most analytes in comparison with the
other matrices. This may be related to the matrix. The validated range of the method is approximately
10 µg/kg to 250 µg/kg for individual alkaloids. Determination of concentrations above 250 µg/kg is possible by applying a higher spiking level and dilution of the sample extract, but this has not been
validated in the collaborative trial.
Cross References:ISO 3696:1987
EN ISO 3696:1995
ISO 5725-5:1998
EN ISO 6498
ISO 6498
All current amendments available at time of purchase are included with the purchase of this document. | File Size : | 1
file
, 3.5 MB |
| ISBN(s) : | 9780580917998 |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 42 |
| Product Code(s) : | 30330820, 30330820, 30330820 |
| Published : | 10/16/2019 |
