CRC SWINE LAB PDF

Preface

This book is an expanded and updated version of Surgery, Anesthesia and Experimental Techniques in Swine which was published in 1998. Over the years I have kept a file of questions and communications concerning the use of swine as animal models that arose from readers of that publication. We also hold annual training classes for swine users which have involved discussion and demonstration of many models and surgical techniques that have not yet been published in another format. Answers to all of the questions I have been asked from these sources are included in this edition. The purpose of the book is to provide a practical technical guide for the use of swine in biomedical research. The primary target audience is investigators, veterinarians, and technicians using swine for experimental procedures. Some of the information may be useful for veterinarians in agricultural practice.

Predominantly this book provides information on models produced by surgical or other invasive procedures. There is a presumption that physicians and scientists will have at least a rudimentary knowledge of the fundamental principles of surgery. It is impossible to fully describe all the models that can be developed in this species; however, there should be enough detail to provide basic principles of performing experiments with an organ or system of interest.

There are new chapters on toxicology and radiobiology that were not included in the previous edition. The toxicology chapter was written by Ove Svendsen, DVM, PhD, from the Royal Veterinary and Agricultural University in Copenhagen, Denmark. Professor Svendsen is recognized as one of the leading authorities on the use of minipigs in toxicology. The number of tables of normal values has been greatly expanded both in the chapters and in the appendix. Dr. Guy Bouchard from Sinclair Research Center supplied much of the information for Yucatan, Hanford, and Sinclair minipigs. Dr. Nanna Grand, Dr. Niels Christian-Ganderup, and Lars Ellegaard of Ellegaard Göttingen Minipigs were very helpful in supplying me with the information on their minipig. Anatomic and physiologic details have also been expanded. Much of this information has never been published in any other format. The sections on anesthesia and perioperative care have been updated substantially. In particular, I have attempted to provide practical information concerning postoperative care for the more complex models. The issues concerning anesthesia and perioperative care have been the primary source of questions I have received over the years.

Of particular note is the addition of magnetic resonance imaging, computerized tomography, and positron emission tomography. The DVD attached to this book contains images collected by these techniques. I owe a great deal of gratitude to my colleagues from the PET Center and Aarhus University Hospitals in Aarhus, Denmark, for this contribution. This effort was led by Aage Kristian Olsen, DVM, PhD, and his colleagues. I owe a debt of gratitude to Jens C. Djurhuus, MD, who helped to coordinate this effort through his position as professor and director of the Institute of Experimental Clinical Research at Aarhus University Hospital (Skejby Sygehus). I would also like to express my appreciation to Frederik Dagnaes-Hansen, DVM, PhD, for continuing to invite me to participate in his training activities in Aarhus as well. This continued collaboration has been very helpful to me.

Michael Sturek, PhD, and colleagues from Indiana University have added a chapter with information on the unique Ossabaw pig along with scanning images that are included on the DVD. This is the first time this information has been published.

Most of this book was written by me as a sole author; however, I received input from my fellow faculty members at the Medical University of South Carolina. In particular, Robert Hawes, MD, from the Digestive Diseases Center added a great deal of detail in the section on endoscopy. Other faculty members in the Department of Comparative Medicine were also helpful to me in this endeavor with advice and editorial comments. Sarah Bingel VMD, PhD, contributed histologic images of normal tissues. Kathy Laber, DVM, MS, and Alison Smith, DVM, aided me with editorial comments. Other colleagues have supplied me with photos from their work and they are credited in the photo captions they supplied.

My primary interest in publishing this textbook is to decrease the learning curve associated with using swine as models. The default terminology and names of structures is based upon veterinary terminology rather than human, which may initially cause some confusion with some, but the meaning should be clear. It is hoped that my ideas and suggestions will contribute to the appropriate and humane use of swine in biomedical research.