DANSK DS/EN ISO 12966-4 PDF

This international standard specifies a method for the determination by capillary gas chromatography (GLC) of fatty acid methyl esters derived by transesterification or esterification from fats, oils and fatty acids. Fatty acid methyl esters from C4 to C26 can be separated by this standard, including trans isomers, saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters. The method is applicable to crude, refined, partially hydrogenated or fully hydrogenated fats, oils and fatty acids derived from animal and vegetable sources, and fats extracted from foodstuffs. The standard is not applicable to di-, tri-, polymerised and oxidized fatty acids and fats and oils. NOTE For the analysis of animal fats the complete elution of all FAMEs should be checked with C26:0 FAME.