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DANSK DS/ISO/TS 20224-2 PDF

DANSK DS/ISO/TS 20224-2 PDF

Name:
DANSK DS/ISO/TS 20224-2 PDF

Published Date:
08/04/2020

Status:
[ Active ]

Description:

Molecular biomarker analysis – Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR – Part 2: Ovine DNA detection method

Publisher:
Dansk Standard

Document status:
Active
Format:
Electronic (PDF)
Delivery time:
10 minutes
Delivery time (for Russian version):
200 business days
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Choose Document Language:
$21.3
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This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of ovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of ovine material derived from sheep (Ovis aries). The target sequence is a partial fragment of the ovine nuclear prolactin receptor short form mRNA gene (PRLR) (i.e. GenBank accession number AF041979.1)[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).


Edition : 20
File Size : 1 file , 2.8 MB
Number of Pages : 26
Product Code(s) : DS-026, DS-026
Published : 08/04/2020

History

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