DANSK DSF/PREN ISO 13136-1 PDF

This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening. This document is applicable to – products intended for human consumption, – products intended for animal feeding, – environmental samples in the area of food and feed production, handling, and – samples from the primary production stage.