ISO/TS 21569-3:2020 PDF

ISO/TS 21569-3:2020 PDF

Name:
ISO/TS 21569-3:2020 PDF

Published Date:
07/01/2020

Status:
Active

Description:

Horizontal methods for molecular biomarker analysis - Methods of analysis for the detection of genetically modified organisms and derived products - Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms

Publisher:
International Organization for Standardization (Technical Standard)

Document status:
Active

Format:
Electronic (PDF)

Delivery time:
10 minutes

Delivery time (for Russian version):
200 business days

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ISO/TS 21569-3:2020 describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.

This document is applicable to the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate quantity and quality of amplifiable DNA from the relevant matrix.


Edition : 2nd
File Size : 1 file , 1.3 MB
Note : This product is unavailable in Russia, Ukraine, Belarus
Number of Pages : 20
Published : 07/01/2020
Same As : ISO/TS 21569-3:2020

History

ISO/TS 21569-3:2020
Published Date: 07/01/2020
Horizontal methods for molecular biomarker analysis - Methods of analysis for the detection of genetically modified organisms and derived products - Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms
$37.2

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