BS EN ISO 17601:2018 specifies the crucial steps of a quantitative real-time polymerase chain
reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA
extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms
that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies
in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA
extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of
sequences is not necessarily linked to living microorganisms and can comprise sequences amplified
from dead microorganisms.
Cross References:ISO 11063
ISO 10381-6
Incorporates the following:Corrigendum, March 2018
| File Size : | 1
file
, 1.4 MB |
| ISBN(s) : | 9780580983023 |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 38 |
| Product Code(s) : | 30359615, 30359615, 30359615 |
| Published : | 03/22/2018 |
| Same As : | BS EN ISO 17601:2018 |
