This powerpoint presentation begins by providing a brief overview of project objectives, methods and results that include the following: Part I - develop a dynamic membrane, including
forming temperature sensitive brushes from Hydroxypropyl
Cellulose on the Cellulose Acetate membrane, Hydrophilic (extended) at low temperatures, and
Hydrophobic (collapsed) at high temperatures;
compare Method 1 to Method 2;
Part II (work done by the Civil Engineering Department at University of Toledo) -
attach antibodies to the brushes using 1-(3-
Dimethylaminopropyl)-3-ethylcarbodiimide; and,
bacteria attach to the antibodies and are detected using a
fluorescent marker that can be visually detected and quantified. Presentation conclusions indicate the following:
we have successfully modified the membrane using
HPC and have shown temperature activation, NIPAAM
can be attached and method needs optimization;
Method 1 vs. Method 2 -
Method 1 activation effect was high but low flux, and
Method 2 activation effect was low but high flux;
antibodies successfully attached using EDC; and,
bacteria were detected using fluorescence. Includes figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 3.9 MB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 25 |
| Published : | 11/01/2008 |
