This powerpoint presentation begins by providing the research objective: to better understand the fate of
manganese - whether from source
water or treatment plant impurities -
under conditions of biological filtration,
and to improve the water industry's
understanding of, and ability to control,
manganese release or pass-through
during biological treatment. Biofilm T-RFLP analysis results:
T-RFLP analysis has been completed on 32 of the 41
samples received, with nine samples failing to amplify by
PCR, apparently due to low DNA concentrations and/or
PCR inhibitors in the DNA;
T-RFLP is continuing on more reference
control strains;
statistical analysis of
the T-RFLP profiles; the PCR tests were completed for Pedomicrobium
and Leptothrix;
quantitation of PCR results in progress; comparison of T-RFLP and PCR shows that T-RFLP
peak 148 is not a reliable predictor of the presence of
Pedomicrobium; comparison of T-RFLP and PCR shows that T-RFLP
peaks 119, 138, and 470 are not reliable predictors of
the presence of Leptothrix; and,
T-RFLP peaks are often characteristic of several
bacteria. The identity of members of the community
contributing to a peak can only be resolved by
specific PCR or 16S rRNA gene clone library
analysis. Includes tables, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 1.5 MB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 18 |
| Published : | 11/01/2009 |
