This powerpoint presentation begins by providing a brief overview of biological perchlorate reduction
and molecular biology tools (MBTs) for treatment processes. Research objectives included: develop quantitative assay targeting
perchlorate-reduction gene;
examine relationship between perchlorate
removal and gene expression levels; and,
apply qPCR and RT-qPCR assays to
perchlorate treatment bioreactor. Research tasks included:
development of quantitative molecular assays for
perchlorate treatment; and,
application of assays to a bioreactor. Conclusions indicated that: qPCR and RT-qPCR assays provide an additional
line of evidence that microbial perchlorate reduction
was occurring in the bioreactor;
cld gene copy numbers generally were higher
when perchlorate removal was higher;
cld gene transcript copy numbers generally were
higher when perchlorate removal was higher; when bioreactor performance declined, gene
copies and gene transcript copies also declined; and, bioreactor failure may be explained, at least
in part, by changes in the microbial community. Includes figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 1.3 MB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 25 |
| Published : | 11/01/2009 |
