Cryptosporidium analyses usually result in a high
incidence of nondetectable samples because of low concentrations
of oocysts and low recovery efficiencies in
environmental water, suggesting greater volumes of
water samples. However, there are limitations in analyzing
high sample volumes, such as low filter performance
and limited volume-processing capabilities when using
US Environmental Protection Agency method 1622.
This study provides guidelines for using water turbidity
measurements to determine optimum sample
volumes for the detection of Cryptosporidium oocysts
using method 1622. The purpose of collecting larger
volumes of water samples is not necessarily to increase
the effective sample volume. The optimum sample
volumes suggested in this article can minimize the
method's limitations and consequently achieve better
recovery of oocysts in water. Use of the optimum-volumes
strategy suggested in this study would save
sample filtration time, enhance filter performance, and
offset the poor recovery efficiency of oocysts that typically
results from higher volumes of water and high
turbidity. Includes 8 references, table, figure.
| Edition : | Vol. 99 - No. 10 |
| File Size : | 1
file
, 100 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 3 |
| Published : | 10/01/2007 |
