This report describes flow cytometric identification, cell and molecular
characterization of two E. intestinalis subpopulations isolated from cell culture
propagated, Percoll-purified spore suspensions. The results of the molecular analysis of both spore subpopulations indicated that both populations possessed
E. intestinalis 16S rRNA and ¿-tubulin genes. However, only one subpopulation
was able to infect RK-13 cells. Researchers should be aware that there is
inherent variability in the viability of microsporidia spores propagated using cell
culture.
Includes 11 references.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 120 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 5 |
| Published : | 09/22/2002 |