The objective of this study was to demonstrate that ribosomal RNA-targeted techniques
could be used to quantify viable microorganisms when determining disinfection efficacy. The
authors have developed a new technique to simultaneously measure ribosomal RNA as well as
precursor ribosomal RNA, a key intermediate in the production of ribosomes. Ribosomal RNA
and precursor ribosomal RNA were measured simultaneously in cultures of Acinetobacter
calcoaceticus<sup>T</sup> and Mycobacterium mucogenicum. The results demonstrate that precursor ribosomal
RNA is a sensitive measure of viability. The authors believe this approach holds tremendous
promise for verifying disinfection efficacy for microorganisms using molecular biology-based
monitoring. In the future, coupling the rapid developments in molecular biology-based technology
with the critical measurement of cellular viability via detection of ribosome genesis may change the
way disinfectants are evaluated. Includes 12 references, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 430 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 8 |
| Published : | 11/02/2003 |
