The main goal of this study was to develop a molecular method alternative to the current
technique, based on immunofluorescence, with the same detection limits, high specificity and
be more secure. This protocol must permit making confident and comfortable decisions in
cases of Cryptosporidium incidents.
This study presents the first results on the development, including the design of probe and
primers with HSP 70 gene as target, the comparison and optimization of two DNA extraction
methods. To build the standard curve, the study prepared a suspension of oocysts purified from
faeces, made a numeration with a solid phase cytometer and performed a DNA extraction to
prepare a stock solution.
With this protocol, the study could detect up to 2 oocysts. The detection of 1 copy is achieved in
60% of the cases.
Currently, the study needs to validate this approach on real samples. Preliminary results are
promising.
Availability of such a tool would allow multiple controls at the different stages of the
water production and possibly the analysis of more complex matrices or coupling this
protocol with an infectivity protocol. Includes 6 references, tables, figure.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 270 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 8 |
| Published : | 11/02/2003 |
