This study reports on a rapid and sensitive method for the determination of six algal toxins that was
developed using liquid chromatography separation with tandem mass
spectrometric detection (LC/MS/MS). Six algal toxins
(Cylindrospermopsin toxin, Anatoxin-a, Nodularin, and Microcystins LR,
YR and RR) were separated using reverse phase chromatography using a
formic acid/methanol gradient. Electrospray ionization in both negative
and positive mode was used for all compounds. For the determination of
soluble algal toxins, two mL of water were filtered through a 0.45 µm
membrane filter then stored at 4°C until analysis. To determine total algal
toxins, 125 mL of sample were frozen at -80°C then thawed three consecutive
times, and then filtered as with the dissolved fraction. An injection
volume of 20 µL was used for all analyses. A reporting limit of 0.50 µg/L
has been established for all target algal toxins. Water samples were
collected from Lake Mead, Nevada where a Cylindrospermopsin algal
bloom had been reported. Despite the fact that the sampling took place
after the bloom had receded, Cylindrospermopsin toxin was still
detectable, yet less than the reporting limit of 0.05 µg/L. An extensive
monitoring program has now been established at Lake Mead, and
preliminary results are presented. Includes tables, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 290 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 6 |
| Published : | 11/15/2004 |
