In this work, a rapid detection method for studying the viability of L.
pneumophila cells combining Propidium Monoazide (PMA) treatment and qPCR has
been developed. This compound specifically intercalates and cleaves the genomic DNA
from dead cells that cannot be amplified by polymerase
chain reaction (PCR). This method,
applied to water samples spiked with L. pneumophila, has demonstrated a signal
reduction of 4.74log if the results are compared with those obtained by qPCR without
PMA, and similar results if compared with the culture isolation method. This
combined assay has also proved to be useful for specific detection of cultivable L.
pneumophila cells from environmental samples. Nevertheless, the qPCR combined with
PMA underestimate the number of viable cells by approximately 0.6 log compared to
culture isolation counts. Includes 9 references, tables, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 810 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 8 |
| Published : | 11/01/2009 |
