This paper addresses some of the inadequacies of the current methods and standards
used for evaluating microbial water quality within drinking water systems and source waters. The
goals were to optimize and implement tangential flow ultrafiltration (TFU) technology for the
simultaneous recovery of low levels of bacteria, viruses, and protozoa from large-volume water
samples; to evaluate quantitative polymerase chain reaction (qPCR) for the detection of select
enteric pathogens; and, to apply these methods to large-volume environmental water samples for
the detection and quantification of microbial contaminants. A TFU method was optimized to simultaneously concentrate and recover each class of
microorganism from 100L water samples. Filter type, surfactant addition, and elution steps were
evaluated during TFU optimization. For these evaluations, microbial surrogates including E. coli
CN-13, E. faecalis, C. perfringens spores, MS2 and PRD1 bacteriophages, poliovirus and murine
norovirus (MNV-1) were added to either dechlorinated tap water (DTW) or surface water (SW).
| Edition : | Vol. - No. |
| File Size : | 1
file
, 830 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 3 |
| Published : | 11/01/2009 |
