In this study, different approaches based on the real-time amplification of different molecular
markers (DNA, rRNA, mRNA) were compared in order to assess the viability status of
Pseudomonas aeruginosa and Escherichia coli cells after heat-killing and chlorine treatments.
The analysis of residual DNA and RNA extracted from water samples after lethal treatment
by electrophoresis bioanalyzer, demonstrated that DNA molecules can survive for up to 24 hours
post death, although RNA (mainly rRNA molecules) started to disappear immediately after
treatment. Disappearance of 48 transcripts was analyzed by two-steps reverse transcription
and real-time PCR (RT-PCR) in starved and not-starved P. aeruginosa and E. coli population.
Results showed rplP, rplV, rplE and rpsD as the most labile transcripts after P. aeruginosa
death, although groEL was the most labile transcript in E. coli. Moreover, some opposite
results were obtained between starved and freshly cultivated E. coli cells. Includes 21 references, table, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 880 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 8 |
| Published : | 11/01/2009 |
