The objectives of this research included: optimization of oocyst removal from U.S. Environmental Protection Agency (USEPA)
Method 1622/23 slides and DNA extraction; development of a PCR-based method for
detection of human infectious and animal related Cryptosporidium genotypes; and, round-robin
laboratory evaluation of the developed method. The use of bacteriological loops
and closed cell foam swabs were compared for their effectiveness in scraping slide
surfaces. Several different types of oocyst lysis and DNA extraction methods were
evaluated. For the detection and genotyping of oocysts, a single round multiplex PCR
targeting the Cryptosporidium genes for 18S ribosomal RNA (18S rDNA) and heat shock
protein 70 (hsp70) was developed. Includes extended abstract only.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 820 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 3 |
| Published : | 11/01/2009 |
