DANSK DSF/FPREN 17641 PDF

This document describes a method using isotopically labelled standards for the quantitative determination of aflatoxins B1, B2, G1, G2 and M1 (AFB1, AFB2, AFG1, AFG2 and AFM1), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), T-2 and HT-2 toxins (T-2 and HT-2) and fumonisins B1 and B2 (FB1 and FB2) in foods by liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS). A specific immunoaffinity column (IAC) clean-up is needed for aflatoxins (AFs) and OTA in food intended for infants and young children (e.g. infant cereals, milk-based powders), in spices, in dried fruits and in nuts. The method has been validated through an intercollaborative study on different commodity groups: cereals and cereal-based products including food for infant and young children, nuts, spices, dried fruits and milk powder. The measuring range of each mycotoxin in these naturally contaminated and/or spiked food samples were: - AFB1: 0,085 7 µg/kg – 11,4 µg/kg; - AFB2: 0,079 2 µg/kg – 12,5 µg/kg; - AFG1: 0,062 8 µg/kg – 20,9 µg/kg; - AFG2: 0,052 0 µg/kg – 15,0 µg/kg; - AFM1: 0,034 2 µg/kg – 0,110 µg/kg; - OTA: 0,448 µg/kg – 17,2 µg/kg; - DON: 45,2 µg/kg – 743 µg/kg; - ZEN: 9,57 µg/kg – 131 µg/kg; - T-2: 10,3 µg/kg – 57,9 µg/kg; - HT-2: 9,50 µg/kg – 81,8 µg/kg; - FB1: 31,1 µg/kg – 4 260 µg/kg; - FB2: 44,2 µg/kg – 1 300 µg/kg. The measuring ranges of the method for each mycotoxin/matrix combination are given in Table 8.