This document specifies the minimum requirements for generating and analysing whole genome
sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting
phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
— products intended for human consumption;
— products intended for animal feed;
— environmental samples from food and feed handling and production areas;
— samples from the primary production stage.
| File Size : | 1
file
, 1.7 MB |
| ISBN(s) : | 9780539050790 |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 58 |
| Product Code(s) : | 30396470, 30396470, 30396470 |
| Published : | 07/05/2022 |
| Same As : | BS EN ISO 23418:2022 |
