Blind trials were performed on an optimized cell culture-immunofluorescence (IFA)
procedure, using the HCT-8 cell line, to determine the sensitivity and reproducibility for
measuring infectivity of flow cytometry prepared inocula of C. parvum oocysts. Using
between 0-100% viable oocysts, prepared at the U.S. Environmental Protection Agency (USEPA), cell culture-IFA was
performed on known oocyst inocula of unknown infectivity at the American Water (AW) laboratory.
Results indicated the control (100% live) oocyst suspensions yielded statistically similar
results to cell culture dose response curves developed previously at AW. For test samples
containing oocyst suspensions of unknown infectivity, cell culture-IFA analyses indicated
a high degree of correlation (r2= 0.89; n=26) with the values expected by USEPA. Cell
culture infectivity correlates well with neonatal mouse infectivity assays and these "blind"
validation trials provide credibility for the cell culture-IFA procedure as a cost-effective
and expedient alternative to mouse infectivity assays for determining in vitro infectivity
of C. parvum oocysts. Includes 20 references, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 290 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 11 |
| Published : | 11/01/2005 |
