This powerpoint presentation begins by providing a brief overview of Cryptosporidium and a choice of detection methods relative to the objectives. Study objectives were to improve Cryptosporidium risk assessment
by optimizing the CC-IFA method via
maximizing infectious foci counts
within a physiological context, and
combining total and infectious counts
on the same sample to remove the bias
induced by non-homogeneous
sample split. Experimental approach used cell cultures and immunofluorescence assays. Results are given for: method optimization; optimizing stimulation media; optimizing infectious media; IMS dissociation media; direct dual detection and
evaluation of oocyst loss; and, impact of reference total count. Conclusions are given for CC-IFA media optimization, and for CC-IFA dual detection. Includes tables, figures.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 1.7 MB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 36 |
| Published : | 11/01/2008 |
