The U.S. Environmental Protection Agency's (USEPA's) National Homeland Security Research Center (NHSRC) is currently working with laboratories and subject matter experts (SMEs) to optimize and verify
culture-based methods for S. Typhi and V. cholerae O1 and O139 in environmental matrices (e.g., drinking water,
surface water). Once the culture component has been optimized, a polymerase chain reaction (PCR) protocol will
be optimized for use with the enrichment step included in each culture method; this will allow laboratories to utilize
the same upfront processing for either culture or molecular analyses. Most of the available culture-based protocols
were not developed or optimized for environmental matrices, requiring some method development work to address
background flora, complex environmental matrices (e.g., surface waters), and spiking procedures to evaluate
recoveries.
| Edition : | Vol. - No. |
| File Size : | 1
file
, 770 KB |
| Note : | This product is unavailable in Ukraine, Russia, Belarus |
| Number of Pages : | 3 |
| Published : | 11/01/2009 |
