Name:
DANSK DS/ISO 21115 PDF
Published Date:
04/12/2019
Status:
[ Active ]
Publisher:
Dansk Standard
This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).
| Edition : | 19 |
| File Size : | 1 file , 4 MB |
| Number of Pages : | 48 |
| Product Code(s) : | DS-041, DS-041 |
| Published : | 04/12/2019 |