DANSK DS/ISO 21115 PDF

DANSK DS/ISO 21115 PDF

Name:
DANSK DS/ISO 21115 PDF

Published Date:
04/12/2019

Status:
[ Active ]

Description:

Water quality – Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

Publisher:
Dansk Standard

Document status:
Active

Format:
Electronic (PDF)

Delivery time:
10 minutes

Delivery time (for Russian version):
200 business days

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This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).


Edition : 19
File Size : 1 file , 4 MB
Number of Pages : 48
Product Code(s) : DS-041, DS-041
Published : 04/12/2019

History


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